The current study has been undertaken to assess knowledge score regarding Impact of mobile phone use on health status among Adolescent boys age between 14-18 years by Information guide sheet. The research design used for study was pre- experimental in nature. The tool for study was self-structured knowledge questionnaire which consists of 2 Parts-Part- I consisted questions related to Socio-demographic data; PART-II consisted of self -structured knowledge questionnaire to assess knowledge score regarding Impact of mobile phone use on health status among Adolescent boys age between 14-18 years. The data was analyzed by using descriptive & inferential statistical methods. After administration of Information guide sheet, some part of population of Adolescent boys (9, 15.0%) Adolescent boys measured with good (13-18) knowledge had aware about Impact of mobile phone use on health status, major part of population of Adolescent boys (51, 85.0%) Adolescent boys measured with excellent (19-24) knowledge had much aware about Impact of mobile phone use on health status and which highlighted the effectiveness of Information guide sheet in terms of better gain in knowledge.