Introduction: Immunization is the process of giving a vaccine to the individual to protect them against various diseases. Vaccines stimulate the body’s own immune system to protect the person against subsequent infection. Children below five year of age are vulnerable and liable to get various diseases and disabilities which may lead to mortality. It is therefore very important to provide care to the children in order to promote the optimal health level.

Aim of the study: Was to improve the knowledge regarding immunization among mothers of under five years children of selected villages at district Mohali, Punjab.

Materials and Methods: A quantitative approach and pre-experimental research design was used to conduct the study. Total 100 samples were selected by using convenient sampling technique. Self-structured Questionnaire was used for data collection and analysis was done by using descriptive and inferential statistics.

Result: The study findings revealed that in pre-test knowledge mean score and standard deviation was 10.18± 2.599. Post-test knowledge mean score and standard deviation was 18.68 ± 3.194.The paired t-test was used in the analysis and p value was 0.001 which is statistically significant.

Conclusion: The conclusion was drawn on the basis of the findings of the study. It reveals that planned teaching program was effective. Our findings highlights need for more intensive efforts to promote proper knowledge regarding immunization schedule.