Materials and Method: A quantitative approach with descriptive design was adapted. The study was conducted in tertiary care hospital. 100 nursing students were selected by using convenient sampling technique. The data was collected by using a Performa of sample characteristics and structured knowledge questionnaire.

Results: The majority of Staff Nurses had average knowledge (40%) regarding prevention of Needle Stick Injuries followed by good knowledge (31%). The mean knowledge score of staff nurses is 18.09+-4.57 regarding the prevention of Needle Stick Injuries. Findings related to association between level of knowledge score and selected sample characteristics: There was no significant association of knowledge score with age, gender, qualification, working experience, area of work, previous history of Needle Stick Injury, attended any health education programme.

Conclusion: This study revealed that undergraduate nursing students had poor knowledge and practice regarding needle stick and sharp injuries during clinical. The most effective means of preventing transmission of blood-borne pathogens is to prevent exposure to NSIs. Primary prevention of NSIs is achieved through the elimination of unnecessary injections and elimination of unnecessary needles. Educational intervention had a positive impact on knowledge and practice of undergraduate nursing students' toward needle stick and sharp injuries.